The Zebrafish Insertion Collection (ZInC) represents a collaborative effort between Shuo Lin's lab at UCLA and Shawn Burgess' lab at NHGRI/NIH to provide freely available zebrafish mutations to the biomedical research community. The labs are working together to generate a genome-wide knockout resource that targets every zebrafish protein-coding gene. They have developed a streamlined pipeline utilizing retroviral mediated insertional mutagenesis together with high-throughput sequencing and mapping technologies. All mutant fish with proviral integrations will be made available to the community through the Zebrafish International Resource Center (ZIRC).
This website provides a searchable interface for determining if a specific gene has been mutagenized by proviral insertion. If the mutant sperm corresponding to that integration site are available from ZIRC, a link to the ordering page will be available and ZIRC will provide mutant fish after the in vitro fertilization. It can take up to six months for sperm to be deposited in ZIRC.
Last modified: Wednesday February 03, 2016