Supplemental Information for:

Human Prostate Cancer and Benign Prostatic Hyperplasia: Molecular Dissection by Gene Expression Profiling1

Jun Luo,2 David J. Duggan,2 Yidong Chen, Jurga Sauvageot, Charles Ewing, Michael Bittner, Jeffrey M. Trent, and William B. Isaacs3

Brady Urological Institute, Johns Hopkins Medical Institutions, Baltimore, MD 21287-2101 [J. L., J.S., C.E., W.B.I.]; and Cancer Genetics Branch, National Human Genome Research Institute, Bethesda, MD 20892-4470 [D.J.D., Y.C., M.B., J.M.T.]

1 Supported by Public Health Service SPORE CA 58236 and DK 52675.
2 These authors participated equally.
3 To whom requests for reprints should be addressed, at 115 Marburg, 600 N. Wolfe Street, Johns Hopkins Hospital, Baltimore, MD 21287. Phone: (410) 955-2518; Fax: (410) 955-0833; E-mail: sisaacs@jhmi.edu.

I.

EXPERIMENTAL DESIGN AND DATA ANALYSIS OVERVIEW. FIGURE 1.JPG

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B.

Complete List of 6500 Human cDNAs, Calibrated Ratios, and Quality Scores. Download Spreadsheet.

Details of Prostate Tissue Sample. View PDF.


II.

MULTIDIMENSIONAL SCALING AND WEIGHTED GENE ANALYSIS. FIGURE 2.JPG

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3-dimensional animation of MDS plot Figure 2A. View QuickTime Animation.

Filtered Gene List. View PDF.

Observed versus Random Sample Permutation Analysis Table. View PDF.

Top 210 Discriminating Genes Ranked by w Values. View PDF.

3-dimensional animation of MDS plot Figure 2C. View QuickTime Animation.

Details of Quality Metric Analysis. View PDF.


III.

HIERARCHICAL CLUSTERING ANALYSIS, GENE DENDROGRAM ANALYSIS. FIGURE 3.JPG

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Dendrogram of Top 210 Discriminating Genes. View PDF.


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VERIFICATION OF DIFFERENTIAL HEPSIN GENE EXPRESSION. FIGURE 4.JPG


V.

RELATED WEBSITES

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NHGRI cDNA Microarray Website: http://www.nhgri.nih.gov/DIR/microarray


 


Supplementary information for Hedenfalk, et. al, New England Journal of Medicine, 244:539-548. (2001)

The New England Journal of Medicine paper: Gene-Expression Profiles in Hereditary Breast Cancer is available here.
The accompanying editorial item by Todd R. Golub, MD, from the same issue is also available here.

NHGRI Homepage          Cancer Genetics Branch        Tissue Microarrays