Table 2. PCR primers used in this study (Sidjanin et. al. 2002, Human Molecular Genetics)*.

A. Primers for amplification of canine CNGB3 cDNA.

Source/target

Name

Sequence 5'->3'

Human CNGB3

CNGB3-2

ATGCCGATGCCCAGCTACACAACCT

Human CNGB3

CNGB3-7

TTGGCAAACCCGTGGGCCACCACAT

Canine CNGB3

CNGB3-16

CTGGATTAAACCCAAAGAAGAGGT

pBSK vector

PBK-III(f)

GGTCGACACTAGTGGATCCAAAG

Canine CNGB3

CNGB3-19

CAGCATCATCAGCAAAGTCGAGTTG

pBSK vector

PBK-VI(r)

CGGCCGCTCTAGAAGTACTCTCG

Canine CNGB3

CNGB3-EX1F

GTTTTCAGACCCACCTCAGA

Canine CNGB3

CNGB3-EX18R

ATCAAAACGGAGGATGGAGG

Canine CNGB3

CNGB3-20

GCCTCGTCTTTCCATATCAA

Canine CNGB3

CNGB3-21

GGACTTCTCCTTGCTTGATG

Canine CNGB3

CNGB3RT-EX5F

CCGTACCATCAACACAAGAG

Canine CNGB3

CNGB3RT-EX7R

TGTAGAGCTCCTGTAGTGTC

B. Primers for BAC 100-02 STSs

BAC 100-O2, end 1 STS

100-02-TJ-1

ATTGCCTGTGGAATCAGGTG

100-02-TJ-2

GCCTAGGACCAGTAATTAGC

BAC 100-O2, end 2 STS

100-02-TV-1

CTGCCTGCTGTACAGAATGTG

100-02-TV-2

GCCCTACAAAGTTAGCAAAGT

STS, 3'UTR canine CPNE3

CPNE3-f1

AGGGAAGGCACATATCCAATT

CPNE3-f2

CCCTAATAAGGTCGAATCTAG

C. Canine CNGB3 primers for exon scanning

Exon 1 

CNGB3-EX1F

GTTTTCAGACCCACCTCAGA

CNGB3-EX1R

ACAAACAGTGCACGACCCAA

Exon 2

CNGB3-EX2F

AAGCTGTGAAGGTCAAGGCT

CNGB3-EX2R

CTTGCATTTTGGCATGTGAT

Exon 3

CNGB3-EX3F

ATAAAATCTCCGAGAAAAAT

CNGB3-EX3R

CTGACTAGGCCTTCTTTCCC

Exon 4

CNGB3-EX4F

CAGTTGCAGAAAGGAGCAGT

CNGB3-EX4R

TTGTCTCAGGGTCTGCTCTT

Exon 5

CNGB3-EX5F

GAAGGGAAGATTCTGACATG

CNGB3-EX5R

AGGGAGAAATGGAAGCCATC

Exon 6

CNGB3-EX6F

TTATCTCCAGAGGACAGTGC

CNGB3-EX6R

CCCTCCTCCATAGTAAATGC

Exon 7

CNGB3-EX7F

TCAGCTGACGTTGCAAGGAA

CNGB3-EX7R

ACTTTAGGTTTGCCTTTGCC

Exon 8

CNGB3-EX8F

ATGGTGCCTGGTCAGCTTAT

CNGB3-EX8R

CAAATTCCATCAGTAGAAACAAT

Exon 9

CNGB3-9F

ACTGGGAGTTTGGCACCATT

CNGB3-9R

CCTGTTCTGAACTGAAGCTG

Exon 10

CNGB3-10F

CCAGTCAAGACACTGCAGTT

CNGB3-10R

CTGGCCATTAATGGGTTGTG

Exon 11

CNGB3-11F

TAAACACCCTCCATGCAGCT

CNGB3-11R

GGCTCATTCACATCCTCTTC

Exon 12

CNGB3-12F

TGTCCTTATGGGGTCAGAAG

CNGB3-12R

TTCCTCAGGAATAGCTGTGG

Exon 13

CNGB3-13F

CACTAGCCATTGTCCAGTTG

CNGB3-13R

GCCATATACAGCAAGAGCTTAG

Exon 14

CNGB3-14F

ACAAGTCCATATCCATTGGC

CNGB3-14R

ATGCTCTTGACCTATGTCTG

Exon 15

CNGB3-15F

GTTCACATGATATGTGCAACC

CNGB3-15R

CCTTTGCCAAGTTTAAGCAG

Exon 16

CNGB3-16F

ACCTGAACTCTCGTCTTTGC

CNGB3-16R

AAGTTGATCCCTGTCATCAC

Exon 17

CNGB3-17F

GGAGAAACATTGGTGTCTCT

CNGB3-17R

GTCTGGGTTTGCTTAGTCCA

Exon 18

CNGB3-18F

GACACTCTTCTCTTGGTGGT

CNGB3-18R

ATCAAAACGGAGGATGGAGG

D. Primers to amplify probes for Southern blot

Exon 18 and 3'UTR

CNGB3 Ex18-F

GAGCCATCAGAGAAAATACTGG

CNGB3 3'UTR-R

CGTGCAAGACTTCAACACAC

Canine CRYBA2

CRYBA2 probe-F

AAGACCCAACTAACATTGATGC

CRYBA2 probe-R

CTCTTAGCACCCCATCACC

*Unless otherwise indicated, primers are based on canine CNGB3 sequence, Genbank accession no. AF490511; human CNGB3 sequence, Genbank accession AF272900.

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