NHGRI Dog Genome Project: Werner 1999

Werner et. al. 1999: Anchoring of Linkage Groups with Chromosome-Specific Markers

This version of the canine linkage map includes 341 mapped (at lod >= 5.0) markers distributed over the X and 37 autosomal linkage groups. The average distance between markers on the map is 9.0 cM, and the linkage groups provide an estimated coverage of over 95% of the genome. Fourteen linkage groups contain either gene-associated or anonymous markers localized to cosmids that have been assigned to specific canine chromosomes by FISH. These 14 linkage groups contain 150 microsatellite markers and allow us to assign 40% of the linkage groups to specific canine chromosomes.

Canine reference pedigrees

A total of 247 animals were genotyped for this study, with a total of 163 F2 individuals. The majority of pedigrees are from the Cornell Canine Reference Families.

The reference for this paper is:

Werner P., Mellersh C.S., Raducha M.G., DeRose S., Acland G.M., Prociuk U., Wiegand N., Aguirre G.D., Henthorn P.S., Patterson D.F. and Ostrander E.A. (1999). Anchoring of canine linkage groups with chromosome-specific markers. Mammalian Genome 10: 814-823

Idiograms

Idiograms of canine chromosomes show the physical localization of gene and anonymous markers as determined by FISH. Existing linkage groups were assigned to chromosomes by incorporating these markers. *Assignment of L1 to CFA1 is based on linkage to marker ZuBeCa2, the physical location of which was demonstrated on CFA1 by Schläpfer et al. (1998). The centromeric end of chromosomes is at the top of each idiogram. Orientation of linkage groups with respect to the centromere is definitive for CFA9, for which two or more FISH probes were used simultaneously, but tentative for other mapped chromosomes. Linkage distances between markers were based on Lod scores of 3 or greater, except for the distance between ZuBeCA5 and C05.771 on CFA5, which was 33.2 cM, supported by a Lod score of 2.5. HSA designations on the linkage maps indicate the chromosomal locations of the corresponding genes on human chromosomes. DGN8 on CFA10 was described by Mariat et al. 1998.